INVESTIGATION OF THE BIOCIDAL EFFECT OF ELECTROCHEMICALLY ACTIVATED AQUEOUS SODIUM CHLORIDE SOLUTION ON STAPHYLOCOCCUS AUREUS

Abstract

Studies were carried out to determine the sensitivity of Staphylococcus aureus to electrochemically activated 3% aqueous sodium chloride solution (anolyte) in different concentrations – 100 %, 50 %, 25 % and 12.5 %. As a control was used the disinfectant Virkon S, applied at final concentrations of 1 %, 0.5 %, 0.25 % and 0.125 %. Two referent strains of S. aureus were used – ATCC and Kowan. It had been found that the anolyte in concentrations of 50 and 100 % inactivates the cells of S. aureus ATCC in suspension at a density of 106 cells/ml within 5 min. After 10 minutes of impact and lower concentrations (25 and 12.5 %) had a bactericidal effect. The anolyte in all tested concentration (12.5 to 100 %) had a bactericidal effect on the cells of S. aureus Kowan in suspension with concentration of 106 cells/ml in 10-minutes. Shorter intervals tested (2 min and 5min) were not sufficient for achieving bactericidal action even at a concentration of anolyte 50 and 100 %, while after 10 min and even smaller concentrations (25 and 12.5 %) had such action. S. aureus ATCC showed slightly higher sensitivity to anolyte and Virkon S compared to the other tested strain Kowan. The effect of the control disinfectant Virkon S on the tested staphylococcal strains was completely analogous to that of the anolyte.