OXIDATIVE STRESS IN BLACK-NECKED PHEASANTS WITH SIGNS OF CANNIBALISM – THE USE OF TRYPTOPHAN AND SILYMARIN AS ANTIOXIDANTS

Abstract

The oxidative stress indicators were studied  Malondialdehyde, Protein carbonyl content, Superoxide dismutase and Catalase in Black-necked pheasants. Blood samples were taken from pheasants, divided into 4 groups (n=12): I group (negative control)  clinically healthy; II group  treated with tryptophan; III group  treated with silymarin; IV group (positive control)  pheasants with signs of cannibalism. Lipid peroxidation were expressed in MDA and the protein oxidation with increased PCC. This was found in the positive control with value for MDA (5.16±0.39) and PCC (7.70±0.45), respectively the negative control (2.35±0.19) and (1.46±0.25). The tendency for SOD was opposite with very high activity in the negative control (2.99±0.10), and in the positive control was (1.64±0.20). Compensatory, the activity of CAT levels in the negative control (1.33±0.24) was higher in comparison to the positive control (6.65±0.73). The silymarin had pronounced anti-oxidant effect on each of the observed indicators, while the effects of tryptophan were in lower rate.