POLYMORPHISM OF ABCG2 GENE AND ITS EFFECTS ON LITTER SIZE AND MILK PRODUCTION OF SYNTHETIC POPULATION BULGARIAN MILK EWES

Abstract

The aim of present study was to investigate polymorphism of ABCG2 (ATP-binding cassette sub-family G member 2) gene and its effect on litter size and milk production. The experiment included 30 ewes of Synthetic Population Bulgarian Milk (SPBM) breed from Institute of Animal Science – Kostinbrod. For genotyping of ABCG2 locus it was used only PCR amplification using specific set of primers. As a result two alleles (D and I) and two genotypes (ID and DD) were established in studied sheep of SPBM breed for locus ABCG2. The frequency of allele D was 0.68 and the frequency of allele I was 0.32. The genotype ID was with frequency 0.63 and genotype DD – with 0.3. In this experiment was studied the relationship between different genotype variants of the tested locus and litter size and milk yield of ewes from Synthetic Population Bulgarian Milk. The comparative analysis was performed using the statistical method ANOVA but no statistically significant differences were observed.