THE EFFECT OF THE DEGREE OF DILUTION ON THE MOTILITY AND VELOCITY PARAMETERS OF SPERM IN RAMS

Authors

  • Hristina Blagova Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences
  • Madlena Andreeva Laboratory of Free Radical Processes, Institute of Neurobiology, Bulgarian Academy of Sciences
  • Rossen Stefanov Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences

DOI:

https://doi.org/10.5281/zenodo.5743820

Keywords:

dilution, motility, velocity parameters, sperm, rams

Abstract

The aim of the present study was to trace the influence of the degree of dilution on the motility and velocity parameters of ram sperm. For this purpose, 12 ejaculates of 6 rams, Northeast Bulgarian fine- fleece breed. Sperm motility and velocity parameters were analyzed by computer sperm analyzer (CASA). Samples were diluted 1: 3, 1: 6, 1: 9, 1:12; 1:24 with extender 6 A. It was found that the total motility (TM) at dilutions from 1: 3 to 1:12 decreased by 2% (P≤0.001), while at 1:24 by 25% (P≤0.001. A significant effect of dilution was found in progressive sperm motility (PM). At dilutions from 1: 3 to 1:12 it decreased by 27.47% (P≤0.001), while at 1:24 the decrease was by 56.63% (P≤0.001). The degree of dilution affects some velocity parameters of the sperm, as Rapid, Medium, VCL, VSL, LIN, STR, WOB and AHL.

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Published

2021-11-27

How to Cite

Blagova, H., Andreeva, M., & Stefanov, R. (2021). THE EFFECT OF THE DEGREE OF DILUTION ON THE MOTILITY AND VELOCITY PARAMETERS OF SPERM IN RAMS. TRADITION AND MODERNITY IN VETERINARY MEDICINE, 6(2), 117–120. https://doi.org/10.5281/zenodo.5743820

Issue

Vol. 6 No. 2 (2021): Volume 6 Issue 2 (2021)

Section

Articles

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Copyright (c) 2021 TRADITION AND MODERNITY IN VETERINARY MEDICINE

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